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Alexander Mankin, Ph.D.Shura Mankin

Professor
Center for Pharmaceutical Biotechnology and
Department of Medicinal Chemistry & Pharmacognosy

University of Illinois at Chicago
Molecular Biology Research Building
900 South Ashland (M/C 870)
Chicago IL 60607-7173

Office: 3052 MBRB
Office Phone: 312-413-1406
Lab Phone: 312-996-4002
Fax Number: 312-413-9303
e-mail address: shura@uic.edu
Mankin Lab homepage: http://mankinlab.cpb.uic.edu/

Education:

Moscow University, Russia D.Sci. (Mol. Biol.) 1989
Moscow University, Russia Ph.D. (Mol. Biol.) 1981
Moscow University, Russia MS (Chemistry of Natural compounds) 1973-1978

Research Interests:

The main interests of this laboratory are functions of ribosomal RNA and mechanisms of action of ribosome-targeted antibiotics. We use a combination of genetic and biochemical techniques to understand how ribosomal RNA participates in protein synthesis and how the ribosome interacts with the newly made polypeptide. We study how antibiotics bind to the ribosome and how they interfere with the ribosome functions. We investigate mechanisms of drug resistance and are trying to develop new superior antibiotics.

Professional Organizations:

RNA Society, American Society for Microbiology

Honorarium:

1998 Teacher of the year award(P1 class)
1999 Teacher of the year award (P1 class)
2001 UIC Center for Excellence in Teaching and Learning, Teacher Recognition Program Award
2005 Excellence in Teaching Award
2005 The University Scholar Award
2006 Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC)award for the best presented work.

Courses:

Pharmaceutical Application of Molecular Biology (PMMP412/MDCH412)
Principles of Medicinal Chemistry (MDCH561)
Fundamentals of Drug Action I (PHAR331)
Principles of Drug Action and Therapeutics VI (PHAR406)
Laboratory Techniques in Pharmaceutical Biotechnology (PMPG522,PMPG523)
Coordinator of the Biotechnology track of Graduate Program in Pharmacognosy

Selected Recent Publications:

  1. Mankin, A.S. (2006) Antibiotic blocks mRNA path on the ribosome. Nat. Struct. Mol. Biol. 13, 858-860.
  2. Tenson, T., Mankin, A.S. (2006) Antibiotics and the ribosome. Mol. Microbiol. 59,1664-1677.
  3. Mankin, A.S. (2006) Nascent peptide in the birth canal of the ribosome. Trends. Biochem. Sci., 31, 11-13, 2006.
  4. Maguire, B.A., Beniaminov, A.D., Ramu, H., Mankin, A.S., Zimmermann, R.A. (2005) A protein component at the heart of an rna machine: the importance of protein L27 for the function of the bacterial ribosome. Molec. Cell, 20, 427-435.
  5. Yassin, A., Fredrick, K., Mankin, A.S. (2005) Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics. Proc. Natl. Acad. Sci. USA. 102, 16620-16625.
  6. Xiong, L., Korkhin, Y., Mankin, A.S. (2005) Binding site of bridged ketolides in the Escherichia coli ribosome. Antimicrob. Agents Chemother. 49, 281-288.
  7. Vimberg, V., Xiong, L., Bailey, M., Tenson, T., Mankin, A.S. (2004) Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel. Mol. Microbiol. 54, 376-385.
  8. Colca, J.R., McDonald, W.G., Waldon, D.J., Thomasco, L.M., Gadwood,, R.C., Lund, E.T., Cavey, G.S., Mathews, W.R., Adams, L.D., Cecil, E.T., Pearson, J.D., Bock, J.H., Mott, J.E., Shinabarger, D.L., Xiong, L., Mankin, A.S. (2003) Crosslinking in the living cell locates the site of action of oxazolidinone antibiotics. J. Biol. Chem., 278, 21972-21979.
  9. Polacek, N., Gomez, M.J., Ito, K. Xiong, L., Nakamura, Y., Mankin, A.S. (2003) The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination. Mol. Cell, 11, 103-112.
  10. Polacek, N., Swaney, S., Shinabarger,D., Mankin, A.S. (2002) SPARK-a novel method to monitor ribosomal peptidyl transferase activity. Biochemistry 41, 11602-11610.
  11. Garza-Ramos, G., Xiong, L., Zhong,P., Mankin, A.S. (2001) Binding site of macrolide antibiotics on the ribosome: new resistance mutation identifies a specific interaction of ketolides with rRNA. J. Bacteriol., 183,6898-6907.
  12. Tenson, T., Mankin, A.S. (2001)Short peptides conferring resistance to macrolide antibiotics. Peptides 22, 1661-1668.
  13. Polacek, N., Gaynor, M., Yassin,A., Mankin, A.S. (2001) Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide. Nature411, 498-501.
  14. Belova, L., Tenson , T., Xiong,L., McNicholas, P.M., Mankin, A.S. (2001) A novel site of antibiotic action in the ribosome: Interaction of evernimicin with the large ribosomal subunit. Proc. Natl. Acad. Sci. USA 98, 3726­3731.
  15. Xiong, L., Kloss, P., Douthwaite, S., Andersen, N.M., Swaney, S., Shinabarger,D.L., Mankin, A.S. (2000) Oxazolidinone resistance mutations in23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action. J. Bacteriol.,182, 5325-5331.
  16. Kloss, P., Xiong, L., Shinabarger, D. and Mankin, A.S. (1999) Resistance mutations in 23S rRNA identify the site of action of protein synthesis inhibitor, linezolid, in the ribosomal peptidyl transferase center. J. Mol. Biol., 294, 93-101.
  17. Khaitovich, P, Mankin, A. S., Green, R., Lancaster, L. and Noller, H. F. (1999)Characterization of functionally active subribosomal particles from Thermus aquaticus. Proc. Natl. Acad. Sci. USA96, 85-90.
  18. Tripathi, S., Kloss, P.S. and Mankin, A. S. (1998) Ketolide resistance conferred by short peptides. J. Biol. Chem. 273, 20073-20077.
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